Use of glnQ as a counterselectable marker for creation of allelic exchange mutations in group B streptococci.
نویسندگان
چکیده
Efficient allelic exchange mutagenesis in group B streptococci (GBS) has been hampered by the lack of a counterselectable marker system. Growth inhibition of GBS by the glutamine analog gamma-glutamyl hydrazide requires glnQ. We have used this phenomenon to create a counterselectable marker system for efficient selection of allelic exchange mutants in GBS.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 71 1 شماره
صفحات -
تاریخ انتشار 2005